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Diffuse-synaptic and axonal immunostaining throughout the brain with variable intensities. Generally weak-moderate but it is strong in the lateral septum, nucleus accumbens shell, ventral pallidum, BNST, globus pallidus, preoptic area, hippocmapus, substantia nigra.
In addition, a subset of neurons exhibit somatic (cytoplasmic) immunostaining, like mitral cells in the olfactory bulb or some neurons in the neocortex layer IV-V.
In addition, a subset of glial cells (possibly astrocytes, with their endfeets) is also immunoreactive throughout the brain; more studies are needed to identify their exact type.
Positive cells and structuresi
Manually selected location of the protein positivity, observed by immunofluorescence staining in mouse brain.