Antibodies are used in a variety of research applications and techniques, such as Western Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, and more. The different conditions in each application and the complexity of biological samples ask for great functionality from the antibodies.

The following pages describe some of the most common techniques.


Enzyme-linked immunosorbent assay

Flow cytometry


Immunoelectron microscopy




Proximity ligation assay

Western blot

References and Links

About the Nobel Prize in Physiology or Medicine 1901:
The Nobel Prize in Physiology or Medicine 1901. (n.d.). Retrieved February 19, 2014, from

A summary of the immune system and the breakthroughs for which the Nobel Prize has been awarded:

Relevant Wikipedia pages:

Antibodypedia - An open-access database of publicly available antibodies and their usefulness in various applications: