The Human Protein Atlas project invites submission of antibodies from both academic and commercial sources to potentially be included in the Human Protein Atlas web portal. Submitted antibodies will be validated by the standard procedures employed by the Human Protein Atlas project, including generation of high-resolution immunohistochemistry images representing a wide spectrum of normal and cancer tissues that are manually annotated. Antibodies that meet the Human Protein Atlas antibody validation criteria may be considered to be included in the Human Protein Atlas web portal.
A submission may range from a single antibody to several hundreds of antibodies. Submitters are requested to fill in a submission form with information regarding e.g. Ensembl identifier of the gene/protein target, basic antibody information (host animal, pAb, mAb, etc), antigen information (target sequence is recommended), purification method, previously tested applications and dilutions, etc. Some information in the sheet is optional.
Conditions & procedure
After submission of the form, the Human Protein Atlas will communicate if the antibodies are suitable for further validation or complete protein profiling and discuss the conditions for a collaboration. This information will be kept confidential by the Human Protein Atlas project. If approved, the submitter will be asked to send vials containing a minimum amount of 50µg or equivalent of each antibody delivered in appropriate tubes (preferably 1-2 ml volume with screw cap) and properly labeled. Received antibodies are tested according to the Human Protein Atlas standard quality assurance procedures, including optimization and staining on tissue microarrays including 44 different normal tissues and 20 types of cancer. The generated immunoreactivity is scored manually and validated in accordance with strict guidelines, including comparison with previous gene/RNA/protein characterization data.
Tissue profiles of successfully approved antibodies may be published on the next following release of the Human Protein Atlas web portal or as otherwise agreed. Antibodies accepted for publication on the Human Protein Atlas may also be analyzed with Western Blot using a standard set of lysates, and used for immunofluorescence on cell lines for determination of subcellular localization of the staining. Product number and link to the provider homepage will be displayed on the Human Protein Atlas for each published antibody. The submitter will get access to the validation data prior to online publication. Academic collaborators can ask to further delay the release of the data and images (beyond six months) if a manuscript based on the results from the protein profiling is being prepared. This is normally not granted for longer than one year. Data on non-approved antibodies will only be communicated to the antibody provider and will not be published.