< No: 13 >

Biomarkers for body fluids

Proteins circulating in blood and those found in other body fluids provide important information about health and disease states on both a systemic and organ-specific level. Antibodies and protein fragments generated in the HPA have been used to develop and apply multiplexed assays for discovering disease-related proteins. Together with technical and biological validation schemes, the studies conducted across different disease areas highlight the value of studying proteins as biomarkers.

Key publication

  • Schwenk JM et al., Antibody suspension bead arrays within serum proteomics. J Proteome Res. (2008)
    PubMed: 18588325 DOI: 10.1021/pr700890b

Other selected publications

  • Schwenk JM et al., Toward next generation plasma profiling via heat-induced epitope retrieval and array-based assays. Mol Cell Proteomics. (2010)
    PubMed: 20682762 DOI: 10.1074/mcp.M110.001560

  • Bachmann J et al., Affinity proteomics reveals elevated muscle proteins in plasma of children with cerebral malaria. PLoS Pathog. (2014)
    PubMed: 24743550 DOI: 10.1371/journal.ppat.1004038

  • Ayoglu B et al., Affinity proteomics within rare diseases: a BIO-NMD study for blood biomarkers of muscular dystrophies. EMBO Mol Med. (2014)
    PubMed: 24920607 DOI: 10.15252/emmm.201303724

  • Byström S et al., Affinity Proteomic Profiling of Plasma, Cerebrospinal Fluid, and Brain Tissue within Multiple Sclerosis. J Proteome Res. (2014)
    PubMed: 25231264 DOI: 10.1021/pr500609e

  • Häussler RS et al., Systematic Development of Sandwich Immunoassays for the Plasma Secretome. Proteomics. (2019)
    PubMed: 31278833 DOI: 10.1002/pmic.201900008

Figure legend: Translational pipeline to discover and validate proteins biomarkers in body fluids.

Key facts

  • Multiplexed immunoassays for protein profiling have been performed across multiple diseases
  • Systematic assays for plasma, serum, and cerebrospinal fluid have been performed, allowing analysis of thousands of protein targets
  • More than 80 peer-reviewed, body-fluid biomarker studies have been published by HPA researchers