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Creation of an antibody resource

All antibodies generated within the HPA effort have been made available to the research community through the Atlas Antibodies portal (www.atlasantibodies.com). All antibodies have been validated by tissue profiling in more than 700 tissue samples and annotated by a certified pathologist. Several international efforts have been initiated to provide antibodies to the research community, and HPA has participated in many of these efforts, including the European Union framework program ProteomeBinders and the U.S. National Institutes of Health Protein Capture Reagents Program.

Key publication

  • Taussig MJ et al., ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome.  Nature Methods (2007)
    PubMed: 17195019 DOI: 10.1038/nmeth0107-13

Other selected publications

Figure legend: The accumulated number of unique antibodies used to perform the protein profiling published in the HPA each year, starting in 2005.

Key facts

  • More than 55,000 antibodies have been generated in-house, and approximately 21,000 have passed the stringent annotation of the HPA program for use in immunohistochemistry
  • More than 10 million tissue and cell images have been generated using this antibody resource
  • The validation data for each antibody is shown on the “antibodies and validation” page of each gene
  • All antibodies used in the HPA program are available to the scientific community through the Atlas Antibodies portal (www.atlasantibodies.com)