The principle of solid-phase DNA sequencing was first described in 1988 based on binding of biotinylated DNA to streptavidin-coated magnetic beads and selective elution of one strand using alkali. Solid-phase methods are now frequently integrated into many nextgeneration DNA sequencing methods as well as numerous molecular diagnostics applications.

Key publication

• Ståhl S et al., Solid phase DNA sequencing using the biotin-avidin system. Nucleic Acids Res. (1988)
  PubMed: 3368314 DOI: 10.1093/nar/16.7.3025

Other selected publications

• Hultman T et al., Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res. (1989)
  PubMed: 2668874 DOI: 10.1093/nar/17.13.4937

• Uhlen M., Magnetic separation of DNA. Nature. (1989)
  PubMed: 2770876 DOI: 10.1038/340733a0

• Hultman T et al., Solid phase in vitro mutagenesis using plasmid DNA template. Nucleic Acids Res. (1990)
  PubMed: 2205837 DOI: 10.1093/nar/18.17.5107

• Uhlén M et al., Semi-automated solid-phase DNA sequencing. Trends Biotechnol. (1992)
  PubMed: 1367934 DOI: 10.1016/0167-7799(92)90169-v

• Holmberg A et al., The biotin-streptavidin interaction can be reversibly broken using water at elevated temperatures. Electrophoresis. (2005)
  PubMed: 15690449 DOI: 10.1002/elps.200410070